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DVT diagnosis


DVT diagnosis

 

Epidemiology

§        1 in 1000

§        Case fatality of 1-5%

§        Very age dependant

Morbidity

§        Post-thrombotic syndrome in up to one third

§        Chronic pain

§        Swelling

§        Ulceration

§        Can occur early or have a latency of up to 10 years

§        28% at 5 years

§        Half this with compression stockings for 2 years

Pre- test probability

§        Active cancer incl. treatment within the previous 6 months                                1

§        Paralysis, plaster                                                                                                  1

§        Bed >3 days, surgery within 4 weeks                                                                     1

§        Tenderness along veins                                                                                        1

§        Entire leg swollen                                                                                                 1

§        Calf swollen >3cm                                                                                                1

§        Pitting oedema                                                                                                     1

§        Collateral veins                                                                                                    1

§        Alternative diagnosis likely                                                                                 -2

 

§        Low risk = 0 (3% risk)

§        Moderate risk = 1-2 (17% risk)

§        High risk = 3 or more (75%)

§        Not recommended for patients with previous VTE/ pregnancy, they should all have imaging

 

D-dimers

§        Fibrinogen is a large glycoprotein with 3 pairs of disulphide bonded polypeptide chains

§        N-terminal, E-domain and 2 outer D-domains

§        Cleavage of a small peptide results in fibrin monomer formation

§        Monomers polymerize and form insoluble fibrin

§        Catalysed by activated factor XIII

§        Plasmin cleaves the polymer to yield soluble fragments

§        Throughout each breakdown step the y-y links which connect the D-domains are retained, so that d-dimers are formed

 

§        D-dimer assays

§        All use monoclonal antibodies

§        Techniques include turbidimetry, latex particle agglutination, fluorescence immunoassay, immunofiltration tests and ELISA

§        ELISA methods are generally more sensitive than the latex agglutination

§        Discrepancies in comparability of various assays

§        Partly explained by using combinations of monoclonal antibodies with differing specifity and affinity

§        Different commercial calibrants

§        Citrated plasma is the sample type of choice, as some X-oligomers may be consumed in clot formation

§        Serum samples could lead to false negative values

§        D-dimers to exclude thrombosis

§        Absence of a rise in d-dimers implies thrombosis is not occurring

§        Negative predictive value – requires highly sensitive tests

§        False positive results are common in hospital patients

§        Age of the clot

§        Return to normal 3 months after a DVT

§        The results of testing after 15 days had a significant effect on the discriminatory value

§        Position of clot

§        20% are isolated calf vein thrombosis

§        Reduced sensitivity in isolated calf vein thrombosis

§        Presumably relates to quantity of clot

§        Heparin use

§        Levels fall after heparin

 

Diagnostic imaging

§        Ascending venography

§        Gold standard

§        Detects both distal and proximal thrombi

§        Diasadvantages

§        Invasive

§        Requires contrast

§        Expensive

§        Failure rates

§        Difficult cannulation esp in swollen legs

§        Contrast preferentially travels in superficial system

§        Intra-observer variability is high

§        Inadequate pelvic imaging because of contrast dilution

§        Ultrasound

§        Inability to compress the vein lumen

§        Sensitivity of 89% for all and 97% for proximal DVT

§        Advantages

§        Simple

§        Quick

§        repeatable

§        can identify alternative diagnoses

§        Serial ultrasound

§        DVTs usually start in the calf, but at time of presentation 80% have more proximal thrombosis

§        Between 3-20% with distal thrombosis at presentation, extend

§        Distal DVT that do not extend rarely result in clinically significant emboli

§        If clinical suspicion high and first scan negative, repeat scan in 1 week will pick up clinically important DVTs

§        Safe to withhold anticoagulation until repeat scan

 

§        Diagnosis of recurrent DVT

§        Abnormalities on US may be detected in up to 70% of patients despite no evidence of recurrent disease in the year following a DVT

§        Cumulative risk of recurrence is 17% at 2 years and 28% at 5 years

§        Compare current US with previous or use venography

 

 

Recommended diagnostic strategy

§        Non-pregnant, 1st event

§        Pretest probability and D-dimers

§        Low PTP and negative d-dimers = no imaging incl. serial

§        In high PTP, need imaging regardless of d-dimer


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