HIV


Haematological features of HIV infection

 

Altered Haemopoiesis             

§        Viral

§        Direct infection of precursor cells

§        Altered production of regulatory cytokines (eg. Overproduction of TNF has an inhibitory effect on marrow progenitor cells)

§        Dysplasia

§        Dyserythropoieis is most common – 50%

§        Abnormal granulocytic and megakaryocytic development – 30%

§        Myelodysplasia in HIV is not considered to be pre-leukaemic

§        Drugs

§        Antiretrovirals

§        Cotrimoxazole / pentamidine

§        Dapsone – AIHA

§        Ganciclovir

 

Thrombocytopenia

§        Common at all stages of disease

§        Often due to immune destruction

§        Increased megakaryocytes / platelet IgG

§        Reduced production

§        Treatment similar to ITP but avoid steroids if possible

§        Hypersplenism

§        Drug toxicity

§        TTP

 

Neutropenia

§        Incidence increases as HIV disease progresses

§        Impaired haemopoiesis main mechanism

§        Anti-neutrophil antibodies found in some patients

§        Poor correlation with counts

§        Generally well tolerated unless <0.5 x 109

§        Treated with GCSF

 

Anaemia

§        Multiple causes

§        Impaired erythropoiesis

§        Drug therapy (zidovudine)

§        Reticuloendothelial iron block due to chronic infections

§        Marrow infiltration

§        Haemolysis unusual (but DAT +ve in 6-43%)

§        B12 deficiency (10-35%)

§        Probably due to malabsorption of B12

§        Partial villous atrophy common

§        Infection (chronic parvovirus)

§   Treatment

§        EPO if level <500 IU/L

§        Transfusion

§        Immunoglobulin can be helpful in parvovirus infection

 

Bone marrow changes

§        Cellularity

§        50% hypercellular

§        15% hypocellular

Dysplasia

§        70% show some dysplastic features

§        Erythroid dysplasia 60%

§        Granulocytic dysplasia 30%

§        Megakaryocytes 30%

 

§        Not generally a preleukaemic state

§        No chromosomal abnormalities

 

§        Histiocytes increased

§        Haemophagocytosis

 

§        Plasma cells increased

§        May be atypical

§        Paraproteiniaemia in 9% of HIV positive (excluding AIDS)

§        Some paraproteins directed against gag and pol

 

§        Fibrosis

§        20-50% of trephines

 

Lupus anticoagulant

§        Prolonged APTT

§        Thrombotic events rare in patients with HIV when compared to other patients with APL antibodies

§        Mechanism unknown

§        Association with opportunistic infection

§        P. carinii

 

AIDS-Related lymphoma

§        Non-Hodgkin’s lymphoma

§        Diffuse large cell (65%)

§        12% Primary intracerebral

§        Burkitt’s (33%)

§        Primary effusion (2%)

§        Castleman’s disease
 

Hodgkin’s lymphoma

§        Increased up to 8-fold

§        Largely EBV related

 

 

% EBV related

Diffuse large cell

80

Primary intracerebral

100

Primary effusion

100

Castleman’s disease

 

Burkitt’s

<50

Hodgkins

Strongly associated

 

Castleman’s disease

Kaposi’s sarcoma herpes virus (also causes primary effusion lymphoma)

Lymphadenopathy with angiofollicular hyperplasia and plasma cell infiltration



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